It is also the first part of a crossover episode with the NCIS spin-off, NCIS: New Orleans with the second part, "Sister City, Part II" as part of NCIS: New Orleans Season 2.
This episode also marks the final appearance of Anton Pavlenko.
When the passengers and fight crew of a private plane belonging to a military tech company are discovered to have been fatally poisoned, the NCIS team investigate what happened and during the investigation, they also form a partnership with their New Orleans counterparts as the two teams attempt to find Abby's missing brother and determine a motive for the poisoning.
- Luca Sciuto, Abby's adopted brother is introduced in person for the first time.
- Tony and Zoe have broken up.
- Scott Bakula, Lucas Black, Zoe McLellan and Shalita Grant are all credited as Special Guest Stars.
- Largely because of how it acted as a crossover episode, it does not lead into the credits of NCIS at the end of the episode. Instead, it immediately starts with Sister City: Part 2 upon the end of the episode.
|Leroy Jethro Gibbs||Mark Harmon||Special Agent in charge of the NCIS Major Case Response Team.|
|Anthony DiNozzo Junior||Michael Weatherly||Senior Special Agent and second-in-command of the NCIS Major Case Response Team.|
|Timothy McGee||Sean Murray (actor)||Special Agent of the NCIS Major Case Response Team.|
|Abigail Sciuto||Pauley Perrette||Chief Forensic Specialist for the NCIS Major Case Response Team.|
|James Palmer||Brian Dietzen||Assistant Medical Examiner for the NCIS Major Case Response Team.|
|Eleanor Bishop||Emily Wickersham||NCIS Probationary Agent.|
|Leon Vance||Rocky Carroll||Director of NCIS.|
|Donald Mallard||David McCallum||Chief Medical Examiner for the NCIS Major Case Response Team.|
|Dwayne Cassius Pride||Scott Bakula||NCIS Special Agent in charge of the NCIS: New Orleans office.|
|Christopher LaSalle||Lucas Black||NCIS Special Agent|
|Meredith Brody||Zoe McLellan||NCIS Special Agent|
|Anton Pavlenko||Lev Gorn||Russian Counselor.|
|Luca Sciuto||Tyler Ritter||Abby's adopted brother.|
|Sonja Percy||Shalita Grant||A former ATF agent who is now an official NCIS Special Agent and who works for the NCIS: New Orleans team.|
|Dalton Greenbrick||Bryan Bat||Blye CFO.|
|Eva Azarova||Cassidy Freeman||A woman who appears in the episode.|
|Blake Huxley||Alex Quijano||Blye Head of Security|
|Kensington Bloom||Wendy Davis||Navy Captain.|
|Heidi Orr||Jamie Gray Hyder||Navy Petty Officer First Class.|
|Feliz Kane||Abraham Amkpa||Navy Petty Officer First Class.|
|Burly Man||Ilia Volok||A man who appears in the episode.|
|Jenner Blye||Samrat Chakrabarti||A man who appears in the episode.|